Short answer: Enantios ROA can measure everything from chiral small molecules with multiple chiral centers to oligonucleotides and peptides — and even up to proteins.
The technique and the measurement are the same, but how ROA results are used in each context is a bit different:
- Chiral small molecules – determine absolute configuration or enantiomeric/diastereomeric excess, without crystallization or experimental references.
- Oligonucleotides & peptides – probe the combination of primary and secondary structure.
- Proteins & ADCs – look at secondary structure and confirm DAR, or structural elements of drug–linker materials when attached to an antibody.
- Stability & degradation – ROA has also been shown in the literature to help determine structural degradation of antibodies and proteins caused by thermal stress.
Want to learn more, or see if our technology can help solve your challenges? Schedule a call with our applications team today at contact@enantios.com.
