Beta-Pinene — Enantiomeric Excess

A series of beta-pinene mixtures across a range of enantiomeric ratios was measured, with ROA peak intensities inverting between the two enantiomers.

Structures of (1R)-(+)-beta-pinene versus (1S)-(−)-beta-pinene

Customer question: I have a mixture of enantiomers — what is the ratio?

  • Enantiomeric excess (EE) determination
  • Typical examples — small molecules with more than two chiral centres

Why come to Enantios?

  • Not enough differentiation with chiral HPLC
  • Reference standards cannot be synthesized, and HPLC requires them
  • A faster, lower-cost solution is needed
Raman spectrum and ROA spectra of beta-pinene mixtures from ee-100 to ee100

Results

ROA spectra clearly distinguish between (+) and (−) enantiomers
Peak intensities inverted between enantiomers
Accuracy achieved: 1.4–5.4% EE
Limit of detection expected down to 0.05%
Fast — enantiomeric excess in as little as 5 minutes
No crystallization or XRD required
Works with experimental or simulated reference spectra

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