Limonene — Absolute Configuration

Chiral identification of limonene with the Enantios ROA solution, with experimental ROA and Raman spectra measured for (S)-(−)- and (R)-(+)-limonene.

Structures of (S)-limonene and (R)-limonene

Customer question: which enantiomer or diastereomer do I have?

  • Absolute configuration (AC) determination
  • Typical examples — small molecules with more than two chiral centres

Why come to Enantios?

  • Molecule cannot be crystallized or separated with HPLC
  • No or only weak differentiation using CD/VCD (if already tried)
    • ORD not expected to be usable for approval
  • Experimental references not available or not easy to synthesize
Experimental ROA spectra of (S)-(−)-limonene and (R)-(+)-limonene with the corresponding Raman spectrum

Results

Clear experimental differentiation of enantiomers
No crystallization or XRD required
Works with experimental or simulated reference spectra
Enantios artifact mitigation and detection ensures clear, consistent results

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